The side also includes West Indies fast bowler Jermaine Lawson, who has been out of competitive cricket since last May because of a suspect bowling action and back problems, and West Indies all-rounder Dave Bernard Jr, who made his debut in both Tests and One-day Internationals last year.
Tikolo and Otieno were two members of the Kenya side that spent the last two months in the Caribbean gaining valuable experience in the West Indies first-class championship.
Tikolo had the second highest aggregate of runs in the Carib Beer 2004 Cricket Series with 382 runs at an average of 31.83 and also captured 15 wickets.
Otieno was the African side's leading batsman with 512 runs at an average of 39.38.
Thursday's match should be the second for Lawson on his return to competitive cricket. He was named in a Jamaica squad to face England in a three-day match, starting on Monday at Sabina Park.
Bernard is an emerging player with a huge reputation in his native Jamaica and played a useful role in helping his national team reach next month's final of the West Indies first-class championship against unbeaten Barbados at Bridgetown.
The rest of the Vice Chancellor's XI comprises mostly young players that have performed with some credit in the Carib Beer 2004 Cricket Series.
England face West Indies in a four-Test series with the first match starting on March 11 at Kingston's Sabina Park.
Kenya to play in WI against England