Already assured of claiming their first interstate title since 1990-91 by posting a mammoth first innings 710, Victoria took giant strides towards a win after leg-spinner Cameron White's three wickets sparked a Queensland collapse of 72 for six just before tea.
Queensland finished the day at 177 for six in their first innings with Martin Love 22 not out and Andy Bichel two not out, still trailing by 533.
Captain Jimmy Maher was out just before stumps, leg before wicket in Jonathan Moss' first over, for 72 after almost four hours of resistance.
Moss then had wicketkeeper Chris Hartley (0) out LBW in his next over. White finished with 3-36 and Moss 2-2.
Although facing a mountain to get within sight of Victoria's total, Queensland were well-placed with openers Maher and Clinton Perren largely untroubled after Victoria was all out just before lunch.
But with the score on 85, Perren (40) got an edge to a Michael Lewis delivery and Victorian captain and wicketkeeper Darren Berry did the rest, completing a great one-handed catch.
White then took two wickets in 10 balls, the first when Stuart Law (18) spooned a wide ball to David Hussey at cover and the second when Chris Simpson (eight) was out LBW.
White later took a third scalp when James Hopes (five) swept across the line and was also adjudged LBW.
Queensland must now survive another two days on a pitch that is starting to keep low and play slow to avoid defeat.
Victoria prolongs the agony of Queensland