Moin stood his ground after being given out leg-before to Irfan Pathan on the fourth day of the match which India won by an innings and 52 runs on Thursday.
"Moin Khan was charged by umpires Simon Taufel and David Shepherd and third umpire Asad Rauf with breaching Section 2.2 of the International Cricket Council's Code of Conduct for showing serious dissent at an umpires decision," a statement from the Pakistan Cricket Board (PCB) said.
"A video tape of the incident was viewed and evidence heard from the umpires who laid the charge. After due consideration of the evidence and the circumstances, match referee Ranjan Madugalle imposed a penalty of 75 per cent of the players match fee," the statement said.
The disciplinary hearing, conducted by Madugalle, was attended by Pakistan captain Inzamam-ul-Haq, manager Haroon Rashid, Moin and the three umpires.
This is the first time in the series, part of India's first full tour of Pakistan since 1989, that a player has been charged with dissent.
Rival captains Sourav Ganguly of India and Inzamam-ul-Haq of Pakistan were earlier fined for slow over-rates during the preceding One-day series.
The remaining two Tests will be played at Lahore from April 5 to 9 and Rawalpindi from April 13 to 17.