The hosts, who under tournament rules have to narrow their squad to 14 in under a month, also included all the players who featured in their lacklustre NatWest Series One-day tournament that concluded on Saturday.
New Zealand beat West Indies in the Lord's final of the triangular event after England crashed out having won just one of their six games.
But among the 10 names unsullied by that reverse are Glamorgan fast bowler Simon Jones and Yorkshire off-spinner Richard Dawson who, while they have both played Test cricket, have yet to make their One-day International debuts.
The other eight new boys are Hampshire seamer Chris Tremlett, Warwickshire batsmen Ian Bell and Mark Wagh, Gloucestershire duo Alex Gidman and Jonathan Lewis, Essex all-rounder Graham Napier and Glamorgan pair Michael Powell and Alex Wharf.
"Our ultimate goal as selectors is to build a side for the World Cup in 2007 and the make-up of this squad gives a good indication as to the type of team we are looking to develop and the particular roles we are looking to fill within it," said chairman of selectors David Graveney.
Wicketkeeper Chris Read, batsman Vikram Solanki and pace bowler James Kirtley all return after touring with England in the off-season despite being overlooked for the NatWest Series.
But Surrey batsman Mark Butcher, 31, was left out. Butcher has played 69 Tests but has yet to appear in a One-day International.
He was due to be called up for the 2002 NatWest Serues before a knee operation ruled him out and Graveney explained: "Mark previously intimated that he did not want to play both forms of international cricket because he was concerned about the impact on his fitness.
"However, he has recently indicated that he would now like to be considered for our One-day side and as the season progresses we will be watching with interest his performances for Surrey in the Twenty20 Cup and the totesport national league."
Michael Vaughan (Yorkshire, capt), Kabir Ali (Worcestershire), James Anderson (Lancashire), Gareth Batty (Worcestershire), Ian Bell (Warwickshire), Ian Blackwell (Somerset), Rikki Clarke (Surrey), Paul Collingwood (Durham), Richard Dawson (Yorkshire) Andrew Flintoff (Lancashire), Alex Gidman (Gloucestershire), Ashley Giles (Warwickshire), Darren Gough (Essex), Stephen Harmison (Durham) Geraint Jones (Kent, wkt), Simon Jones (Glamorgan), Robert Key (Kent), James Kirtley (Sussex), Jonathan Lewis (Gloucestershire), Anthony McGrath (Yorkshire), Sajid Mahmood (Lancashire), Graham Napier (Essex), Michael Powell (Glamorgan), Chris Read (Nottinghamshire, wkt), Vikram Solanki (Worcestershire), Andrew Strauss (Middlesex), Chris Tremlett (Hampshire), Marcus Trescothick (Somerset), Mark Wagh (Warwickshire) Alex Wharf (Glamorgan).