Put in to bat, the hosts managed just 170 for seven in their stipulated 50 overs with Jaya Sharma (43) and Anjum Chopra (26) being the main contributors with the bat.
Keightley had also shone with the ball taking three for 17 and she was well supported by Cathryn Fitzpatrick (2/23).
Two useful partnerships, first a 61-run stand for the opening wicket with skipper Blenda Clarke (23) off 16.2 overs and then the match-winning 90-run partnership for the secondwicket with Rolton off 23.4 overs, not only gave Keightley the Woman-Of-The-Match award but also helped her team take a comfortable 3-1 lead in the seven-match series.
The Aussies scored 173 for two in 44.5 overs.
Brief scores: India 170 for seven in 50 overs (J Sharma 43, A Chopra 26, L Keightley 3/17, C Fitzpatrick 2/23) lost to Australia 173 for two in 44.5 overs (L Keightley 80, K Rolton52, B Clarke 23).