England captain Vaughan, who'd been off the field here Thursday with a recurrence of his longstanding right knee injury while playing for Yorkshire, did not take the field when Sussex's innings in this County Championship match resumed here Friday at Arundel.
He did return to bat, down the order at number seven in Yorkshire's second innings, but managed just four before being lbw fourth ball to Pakistan seamer Yasir Arafat.
It was later announced that the Yorkshire and England medical staffs would be holding a meeting early next week to discuss the best way forward for Vaughan in his recovery.
That seemed to close the door on the possibility of the 31-year-old batsman playing in the final two One-day matches against Sri Lanka at Old Trafford on Wednesday and at Headingley three days later.
He was already set to miss Yorkshire's C and G Trophy One-day match against Worcestershire at New Road on Sunday and is now doubtful for Tuesday's opening Twenty20 match against Durham at Headingley.
England will want Vaughan to prove his fitness in a series of Championship matches before selecting him for the start of the four-Test series against Pakistan at Lord's on July 13.
Vaughan has not played for England since the tour of Pakistan before Christmas. He only returned to cricket with Yorkshire a month ago and has repeatedly used ice treatment on his knee.
England are currently 2-0 down in their One-day campaign against Sri Lanka, meaning they must win at the Riverside on Saturday to keep the five-match NatWest Series alive.