The trial, which had been recommended by a slender 6-5 margin at the ICC cricket committee earlier this year, was rejected on the basis of the impact it may have on the authority of the umpire and the 'spirit of cricket'.
"The reservations expressed by the cricket committee when they recommended the player appeal measure were mirrored to a much greater degree by the ICC board in its rejection of the concept after extensive deliberation," said ICC president Ehsan Mani.
"The board was concerned about the impact of the trial on the spirit of cricket and the effect it might have on the integrity of umpiring at all levels.
"It was also felt the ICC Champions Trophy was too high profile an event at which to undertake such a trial.
"As such, further discussions will now take place to see whether the concept can be tested at domestic level," Mani added.
England coach Duncan Fletcher had repeatedly spoken in favour of the 'three appeal' concept. But, for the time being at least, technology will only be used at the discretion of the on-field umpires.
Meanwhile, another decision from what will be a week of ICC meetings in London, saw the chief executives' committee (CEC) also approved a series of recommendations concerning the issue of bad light in international cricket. Research on the subject will be undertaken in Australia, England and Pakistan and pending the completion and review of that research, artificial lights can still be used in Test matches.
The CEC also agreed to the increased use of light meters as a guideline for determining whether light is fit or unfit for play.
At the ICC annual conference on Friday, Percy Sonn of South Africa is due to take over from Pakistan's Mani as ICC president.
The Champions Trophy, a one-day tournament which is effectively a 'mini World Cup', gets underway in India in October.