Pakistan won the toss at Trent Bridge, Nottingham and opted to bat.
The home team, 2-0 down in the five-match series, also dropped all rounder Rikki Clarke, bringing in Ed Joyce and Michael Yardy as replacements.
Trescothick has had a poor series against Pakistan and was bowled for a first-ball duck in the third one-dayer. He asked not to be considered for the Champions Trophy to continue treatment for a stress-related condition which also cut short his tour to India in February.
The limited-overs tournament will be held in India in October and November.
England: Ed Joyce, Andrew Strauss (captain), Ian Bell, Kevin Pietersen, Paul Collingwood, Jamie Dalrymple, Michael Yardy, Chris Read, Jon Lewis, Sajid Mahmood, Stuart Broad.
Pakistan: Shoaib Malik, Mohammad Hafeez, Younis Khan, Inzamam-ul-Haq (captain), Mohammad Yousuf, Abdul Razzaq, Shahid Afridi, Kamran Akmal, Rana Naved-ul-Hasan, Mohammad Asif, Shoaib Akhtar.