England recalled Andrew Strauss in place of Ed Joyce with Australia bringing in batsman Brad Hodge for injured all-rounder Shane Watson.
England, after their 5-0 Ashes thrashing in Australia, won the triangular one-day series with a 2-0 win in the final against world champions.
In all they have won their last three full one-dayers against Australia but went down to a five-wicket defeat against their oldest rivals in a World Cup warm-up in St Vincent on March 9.
Australia have won all five of their matches at this tournament so far as they chase an unprecedented third straight World Cup title and top the standings in the Super Eights.
England, handicapped by an opening match defeat against New Zealand, are currently in fifth place.
Australia: Adam Gilchrist (wk), Matthew Hayden, Ricky Ponting (capt), Michael Clarke, Andrew Symonds, Michael Hussey, Brad Hodge, Brad Hogg, Nathan Bracken, Shaun Tait, Glenn McGrath
England: Ian Bell, Michael Vaughan (capt), Andrew Strauss, Kevin Pietersen, Paul Collingwood, Andrew Flintoff, Ravi Bopara, Paul Nixon, Sajid Mahmood, James Anderson, Monty Panesar