India, down 2-0, are hoping Sachin Tendulkar's 400th one-day appearance will inspire them to a series-levelling win.
Tendulkar hit a one-day record 84th fifty to lift the hosts to an eight-run victory on Monday as the hosts pulled back to a 2-1 deficit in the seven-match series.
The series opener was washed out.
Both teams retained the eleven that played in the previous game in Chandigarh.
Tendulkar, 34, who holds the one-day record for most runs (15,563), centuries (41) and fifties (84), becomes the second player after Sri Lanka's Sanath Jayasuriya to play in 400 one-dayers.
India: Mahendra Dhoni (captain), Sachin Tendulkar, Saurav Ganguly, Robin Uthappa, Rahul Dravid, Yuvraj Singh, Irfan Pathan, Harbhajan Singh, Murali Kartik, Zaheer Khan, Rudra Pratap Singh.
Australia: Ricky Ponting (captain), Adam Gilchrist, Matthew Hayden, Michael Clarke, Andrew Symonds, Brad Hodge, James Hopes, Brett Lee, Brad Hogg, Nathan Bracken, Mitchell Johnson.