Mohd Kaif has been named as Sachin's replacement for the Ahmedabad Test starting Apr 3.
Kaif, who led his provincial side UP to Ranji Trophy final, played his last Test against the West Indies at Sabina Park in 2006.
He was sidelined after a prolonged poor run with the willow in both the form of the sport.
The star batsman, who needs 172 runs to take over from West Indian Brian Lara as Test cricket's leading run-scorer, did not field for most of the final day's play in the first Test which ended in a draw here.
Former Indian physiotherapist John Gloster had recommended a three-week rest for Tendulkar after the Australian tour ended on March 4.
The 34-year-old, however, declared himself fit for the South African series.
Tendulkar, who fell for zero in India's only innings here, has 11,782 runs from 147 Tests, second behind Lara's record tally of 11,953 runs.