Sharath, who has been a star player in Karnataka's campaign with 32 wickets so far, will miss the Punjab match due to right shoulder injury.
The right-arm fast bowler, who is the second highest wicket-taker this season for the team, injured his shoulder while fielding in the quarter-final against Uttar Pradesh last week at M Chinnaswamy Stadium.
Along with Sharath, there is also one more player who will miss the trip to Punjab. Batsman R Samarth, who injured his left little finger against Uttar Pradesh, has been left out of the 15-man squad.
Left-arm paceman S Aravind and batsman Mayank Agarwal are the replacements for the duo. Aravind has played three matches while Agarwal has featured in seven this season.
Karnataka are on a roll in this season's Ranji Trophy, having notched up six victories in a row.
R Vinay Kumar (captain), Robin Uthappa, KL Rahul, Mayank Agarwal, Manish Pandey, Karun Nair, CM Gautham (vice-captain & wk), Shreyas Gopal, Abhimanyu Mithun, Ronit More, Abrar Kazi, KP Appanna, Ganesh Satish, Amit Verma, S Aravind. Batting coach: J Arun Kumar. Bowling coach: Mansur Ali Khan. Manager: B Siddaramu.